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Reovirus genomic RNA induces IRF3 phosphorylation in SVECs. (A) SVECs were transfected with 0.25 μg or 0.5 μg of rsT1L or rsT3D genomic RNA or with 2.0 μg of poly(I·C). (B) SVECs were transfected with siRNA specific for MAVS or TLR3 alone or in combination for 48 h prior to transfection with 0.5 μg of genomic RNA or poly(I·C) (2 μg). At 6 h, immunoblot analysis was performed for phosphorylated IRF3 (p-IRF3), total IRF3, β-actin, or MAVS. **, P < 0.001 (as determined by Student's t test).
