Figure 2.

Accumulation of JA (A), JAME (B), OPDA (C), OPDAME (D), and α-LeA (E) in barley leaf segments after floating on 1 m sorbitol solution (▪) or distilled water (●) for various times. At each time point, 1 g fresh weight of leaf segments was taken and subjected to GC/MS-SIM analysis (A–D) or GC analysis (E), as described in “Materials and Methods.”