Figure 3.

Northern-blot analysis of mRNA accumulation of jip23 (A–C) and jrg5 (A′–C′) in barley leaf segments treated with 50 μm each of JA, JAME, OPDA, OPDAME, or JA-l-Ile and 10 nm coronatine either for various times (A and A′) or with different concentrations for 24 h (B and B′). Total RNA (10 μg per lane) was used for electrophoresis followed by northern-blot analysis (A and B) and quantification (C and C′) via the phosphor imager system described in “Materials and Methods.”