Figure 5.

Kinetics of mRNA accumulation of JIP-23 during treatment of barley leaf segments with 1 m sorbitol solution. In A, leaf segments were floated continuously on sorbitol. In B and C, leaf segments were floated on sorbitol for indicated times followed by floating on water up to 8 h (B) or 24 h (C). Total RNA (10 μg per lane) was used for northern-blot analysis as described in “Materials and Methods.” Each loading control is given by ethidium bromide staining.