FIG 7.

HAdV-5p, but not HAdV-41p, is neutralized by HD5 and preferentially infects goblet cells. (A) Human enteroid monolayers were infected at MOIs of 30 and 200 for HAdV-5p and HAdV-41p, respectively, in the presence or absence of 15 μM HD5. Infected cells were enumerated by flow cytometry at 48 h postinfection. Data are from four individual replicates graphed as the percentage of HAdV infection in the presence of HD5 compared to infection in the absence of HD5. Bars are means ± SD. (B) HAdV-5p- and HAdV-41p-infected cells from the samples in panel A were analyzed for the percentage of MUC2⁺ goblet cells (GC) and MUC2⁻ nongoblet cells (non-GC) that were infected in the absence of HD5. (C) Data from panel A analyzed as the ratio of HAdV-5p infected GC to non-GC as a function of %GCs. (D to G) Representative flow cytometry plots. For panel A, the percentages of cells infected under each condition were compared. For panel B, the percentages of infected GC and non-GC were compared by ratio paired t tests. *, P < 0.05; ns, not significant.