FIG 5.

Effect of IFN-β on DNA replication. (A) Viral DNA replication. A549 cells were untreated or treated with 2,000 IU/ml of IFN-β for 24 h and then infected with 3 PFU/cell of purified WR or vΔC9. At 0, 2, and 6 h after infection, DNA was extracted with a DNeasy blood and tissue kit (Qiagen). Viral DNA was quantified by ddPCR using primers for the E11L ORF. (B) Plasmid replication. A549 cells were transfected with pUC19 DNA. After 6 h, the cells were untreated or treated with IFN-β for 18 h and mock infected or infected with purified WR or vΔC9. At 6 h after infection, total DNA was isolated and digested with the BamHI and DpnI enzymes. The unmethylated pUC19 DNA was quantified by ddPCR. The experiments were repeated three times, and the data were combined to make the figure. The error bars represent SEMs.