Fig. 2.

MAA antigen in precision-cut liver slices (PCLSs) incubated with ethanol. PCLSs were incubated with control and ethanol-containing media for 3 days, and homogenates [PCLS antigens (PCLSA)] were prepared and subjected to immunoprecipitation using a polyclonal rabbit (Rb) anti-MAA antibody. Western blot using the monoclonal mouse anti-MAA antibody shows unique bands in the PCLSs incubated with ethanol. Bands (black rectangle) from control and ethanol-treated PCLSs were cut out and sent for mass spectrometry to Applied Biomics. Ethanol-treated PCLSs had a positive hit for proteasome subunit β, putative trypsin-6, and glial fibrillary acidic protein. Gels are representative of 5 separate samples, one of which was used for mass spectrometry. Proteins were identified as significant if an 88–99% confidence ID [confidence interval (CI)] score was obtained. MW STD, molecular weight standard.