Fig. 1.

β-PIX (p21-activated kinase-interacting exchange factor) and GIT1 (G-protein-coupled receptor kinase-interacting protein 1) protein expression and their interactions in different lines of intestinal epithelial cells (IECs). A: representative immunoblots of β-PIX, GIT1, and Rac1 in IEC-6 (undifferentiated IECs), IEC-Cdx2L1 [differentiated IECs (line of IEC-Cdx2L1)], and Caco-2 cells. Levels of total β-PIX and GIT1 were examined by Western blot analysis, and GAPDH immunoblotting was performed as an internal control for equal loading. B: levels of GIT1 and β-PIX in materials immunoprecipitated (IP) by the anti-β-PIX antibody (Ab) in cells described in A. After whole cell lysates (400 µg) were IP by the specific antibody against β-PIX, precipitates were separated by performing SDS-PAGE gels. Levels of GIT1 and β-PIX were measured using Western blot analysis with specific antibodies. Three separate experiments were performed that showed similar results.