Fig. 4.

CSF1-Fc treatment causes lipid accumulation in livers of neonatal rats. Rats were injected with PBS or DEX on days 14–21 of pregnancy. Control dams were not injected. Offspring were treated with PBS or 1 µg/g porcine CSF1-Fc on days 0–5. A: representative hematoxylin and Eosin (H&E) sections of formalin-fixed paraffin-embedded livers. ImageJ was used to quantify the percentage area of lipids (B) and lipid size (C), using 6 H&E images per liver; n = 7 (control), 11–12 (PBS), and 7 (DEX). D: representative Oil red O staining of frozen liver sections. E: EDTA-plasma was tested for bile acids; n = 7–8 for all groups. All results were analyzed with a Mann-Whitney test. P values are indicated on the box and whisker plots.