Fig. 5.

Treatment of neonatal rats with CSF1-Fc causes an increase in liver and splenic macrophages. Rats were injected with PBS or DEX on days 14–21 of pregnancy. Control dams were not injected. Offspring were treated with PBS or 1 µg/g porcine CSF1-Fc on days 0–5. Organs were collected at day 6. Representative images of formalin-fixed, paraffin-embedded livers (A) and spleens (B) stained with an antibody against CD68. Sections shown are from neonatal rats born to PBS-injected dams. Percentage area of CD68⁺ staining was calculated with ImageJ using 6 images per organ; n = 6–8 for all groups. All results were analyzed with a Mann-Whitney test. P values are indicated on the box and whisker plots.