Pulmonary arterial myocytes had 3 temporally distinct types of Ca2+ signaling events: Ca2+ sparks, medium-duration oscillations, and long-duration oscillations. A–D: absolute counts of each type of event exposed to DMSO (control), 1 µM FPL 64176 (FPL), or 1 µM FPL with 10 µM NIF for recordings from arteries isolated from each animal group. E–G: representative tracings from the AN control group of a Ca2+ spark, medium-duration oscillation, and a long-duration oscillation, respectively. In all groups, the majority of events were medium-duration oscillations.