Fig. 9.

FPL 64176 (FPL) alone failed to substantially modify Ca²⁺ spark activity. A: percentage of cells with Ca²⁺ sparks. B: Ca²⁺ spark firing frequency when exposed to DMSO (control, closed bars and circles), 1 µM FPL (open bars and circles), or 1 µM FPL with 10 µM nifedipine (NIF) (gray bar, diamonds) for arterial myocytes from adult and fetal sheep under normoxic and hypoxic conditions. Values are means ± 95% CI. Data in A were analyzed by a χ² test. **P < 0.01, ***P < 0.001, while data in B were analyzed by Kruskal-Wallis one-way ANOVA with Dunn's multiple comparison test for each group ⁺P < 0.05, ⁺⁺⁺P < 0.001. Control responses were obtained from 55/141/3 AN, 142/167/3 AH, 19/153/3 FN, and 20/119/3 FH sparks, lines, and animals, respectively. FPL responses were obtained from 14/122/3 AN, 110/183/3 AH, 11/158/3 FN, and 5/103/3 FH sparks, lines, and animals, respectively. FPL with NIF responses were obtained from 14/133/3 AN, 280/216/3 AH, 16/152/3 FN, and 3/106/3 FH regions of sparks, lines, and animals, respectively.