Fig. 3.

Body composition and plasma analysis of male (n = 8) and female (n = 7) PTP1B^flox/flox and male (n = 5) and female (n = 7) PTP1B^flox/flox/POMC-Cre mice during high-fat diet (HFD) feeding. Body weight and food intake were measured twice weekly. EchoMRIs for lean and fat mass were conducted once per week for the duration of the study. A: body weight (g) from 6 to 20 wk of age. B. body weight in male and female PTP1B^flox/flox and PTP1B^flox/flox/POMC-Cre mice at 20 wk of age. C: total fat mass (g) as measured by EchoMRI (g). D: total lean mass (g) as measured by EchoMRI. E: total fat mass in male and female PTP1B^flox/flox and PTP1B^flox/flox/POMC-Cre mice at 20 wk of age. F: average daily food intake (g). G: cumulative food intake from 10 to 14 wk (g). H: plasma leptin (ng/ml). I: plasma insulin (ng/ml). Data are expressed as means ± SE. *P < 0.05. A, C, D, and F: 2-way ANOVA with post hoc Sidak’s multiple comparison; t-test following linear regression analysis. B, E, G, H, and I: unpaired Student’s t-test.