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. 2018 Apr 1;28(2):86–96. doi: 10.1089/nat.2017.0694

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© Colton M. Miller et al. 2018 Published by Mary Ann Liebert, Inc.

This article is available under the Creative Commons License CC-BY-NC (http://creativecommons.org/licenses/by-nc/4.0). This license permits non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. Permission only needs to be obtained for commercial use and can be done via RightsLink.

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FIG. 5. — Malat-1 expression analyzed through qPCR based on PS-ASO against Malat-1 in conjunction with siRNA treatment and DHA media supplementation for LBPA overexpression analysis. cDNA was normalized to ∼50 ng/reaction. Expression of Malat-1assessed by SYBR green qPCR amplification with beta-actin controls. (A) Expression of cells with no siRNA treatment, (B) Expression of cells treated with a negative control scrambled siRNA, (C) Expression of cells treated with siRNA for EEA1, (D) Expression of cells treated with siRNA for RAB7A. Bars represent normalized Malat-1 expression with mean ± standard deviation, n = 3. Statistical analysis was performed using two-tailed Student's t-test P < 0.05.