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Western blot analysis of capsid in virion and producing cells. 293T cells were transfected with pNL-Luc-R-E- plasmid (1L) or mutants 40L, 42L and 47L. Cell lysate of 293T cells and supernatant was subjected to Western blot and capsid proteins were visualized by anti-CA monoclonal antibody. The amounts of p24 antigen in the supernatant and luciferase counts (RLU) in the transfected cells are shown. The experiments were performed in duplicate.
