Table 1.

Primers for PCR amplification and qPCR reaction

Organism	Target gene	Primer	Sequence (5'-3')	Reference
Bacteria	16S	338f	ACTCCTRCGGGAGGCAGCAG	[19]
		806r	GGACTACCVGGGTATCTAAT
R. haemaphysaloides	Actin gene	Ractin-F	GTGCCCATCTACGAAGGTTAC	This study
		Ractin-R	CCATCTCCTGCTCGAAGTCC
Rickettsia-like endosymbiont	Citrate synthase gene (gltA)	gltA-F	TCCTACATGCCGACCATGAG	[20]
		gltA-R	AAAGGGTTAGCTCCGGATGAG
Coxiella-like endosymbiont	16S rRNA gene	L-CoxF	TGAGTGTTGACGTTACCCACAG	This study
		L-CoxR	GCATTTCACCGCTACACCG