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. 2018 Apr 13;9:104. doi: 10.1186/s13287-018-0850-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Confocal laser scanning microscopy analysis. Left panels: Human gingival mesenchymal stem cells (hGMSCs) anti-ANA positive nuclei in a 3D-PLA + hGMSCs, b 3D-PLA + extracellular vesicles (EVs) + hGMSCs, and c 3D-PLA + polyethyleneimine (PEI)-EVs + hGMSCs (green fluorescent). Middle panels: Confocal contrast image of 3D-PLA (gray) grafted in mice calvaria after 6 weeks of implantation. Right panels: Merged images of the two abovementioned channels. Scale bars = 10 μm. *, scaffold; C, rat calvaria