Fig. 2.

Knockdown of TDP43 inhibits breast cancer progression. (A) Cell-growth inhibition upon TDP43 knockdown by shRNAs. (B) Cell-cycle analysis after knockdown of TDP43. (C) Western blotting of cell-cycle and apoptosis-related proteins. (D) Tumor size after 2 mo of mammary fat pad growth in the xenograft assay. Each experimental group contained six mice. (E) Cell migration and invasion levels by Transwell assays. (F and G) Fluorescence density (F) and statistical analysis (G) of metastasis ability by tail-veil injection using 4T1 cells expressing luciferase (n = 9). Data in A–D are shown as the average ± SEM for three independent measurements; ***P < 0.001. All data are for MDA-MB231 cells, except where labeled.