Fig. 3.
In vitro activity of IpdAB. (A) HPLC trace of in vitro transformation of COCHEA-CoA with IpdAB and FadA6. Reaction conditions were 100 μM COCHEA-CoA, 1 μM IpdABRHA1/IpdABMtb, 5 μM FadA6, and/or 125 μM CoASH (10 mM sodium phosphate, pH 8.0). Identified species correspond to CoASH (1), acetyl-CoA (2), COCHEA-CoA (3), and MOODA-CoA (4). (B) Steady-state analysis of IpdAB transformation of COCHEA-CoA [Hepes, pH 7.5, and1 mM MgCl2 (I = 0.01 M) at 25 °C]. Curve indicates least-squares fit of Michaelis–Menten equation to the data.