Table 1.

Steady-state kinetic parameters

Enzyme	Substrate	kcat, s−1	Km, μM	kcat/Km (×104 M−1⋅s−1)
FadA6Mtb*	Acetoacetyl-CoA	4.8 (0.8)	300 (100)	1.6 (0.8)
IpdABRHA1†	COCHEA-CoA	5.8 (0.8)	120 (40)	5 (2)

^*Hepes, pH 7.5, and 10 mM MgCl₂ (I = 0.05 M), 25 °C.

^†Hepes, pH 7.5, and 1 mM MgCl₂ (I = 0.01 M).