Table 2.

Specific activity of IpdAB variants

IpdABRHA1 variant	Relative activity, %
WT	100 (25)
E58BA	80 (20)
E105AA	<0.5
E105AD	<0.5
R126BM	<0.5
R92BM	<0.5

Using 50 μM COCHEA-CoA, 50 μM CoASH, and 2 μM FadA6 [Hepes, pH 7.5, and 1 mM MgCl₂ (I = 0.01 M), 25 °C].