Fig. 2.

cpSRP43 and GBP are cooperatively involved in the stabilization of GluTR in Arabidopsis. (A) Pale-green leaf phenotype of 24-d-old gbp, chaos, and chaos/gbp mutants and their WT plants (Col-0 ecotype for gbp and Ler-0 ecotype for chaos). (Scale bars: 1 cm.) (B) Steady-state levels of TBS enzymes, cpSRP components, and LHC proteins in seedlings of the genotypes mentioned above were quantified by immunoblotting using the indicated antibodies. Ponceau S-stained RubisCo (RbcL) is shown as a loading control. Relative ALA synthesis rates (C), Chl contents (D), and heme contents (E) are shown. Data are mean ± SD (n = 3). In C–E, asterisks indicate significant differences compared with WT plants (*P < 0.05, **P < 0.01; Student’s t test).