Fig. 3.

Properties of ABD–immunotoxin fusion protein. (A) Binding of LMB-12, LMB-164, LMB-20, LMB-182, LMB-170, and LMB-172 proteins to MSA, HSA, and BSA. Microtiter plates were coated with MSA, HSA, or BSA in PBS at 4 °C; diluted fusion immunotoxins were added; and bound immunotoxin were measured by ELISA. (B) Pharmacokinetics of LMB-12, LMB-164, LMB-20, LMB-182, LMB-170, and LMB-172 in mice. Nu/nu mice were injected i.v. with 25 μg of fusion protein. Blood samples were drawn at different times. The levels of immunotoxin in mouse blood were measured by ELISA.