Fig. 4.

Effect of albumin on activity of LMB164: cell-killing curve of LMB164 in the presence of HSA (A) and in the presence of MSA (B). (C) HSA uptake: KLM1 cells were treated with 10 μg/mL HSA-alexa488 with or without 100 ng/mL of LMB-164 for the indicated time. Relative fluorescence intensity was measured by flow and expressed as geomean subtracted background without HSA–Alexa-488. (D) LMB-164 uptake: KLM1 cells incubated with LMB164–alexa-647 at indicated concentration with or without 10 mg/mL of HSA for 3 h. Fluorescence intensity used geomean subtracted from the background cells without LMB164–Alexa-647.