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. 2018 Mar 26;115(15):E3388–E3397. doi: 10.1073/pnas.1722452115

Fig. 7.

Fig. 7.

TET1 and GSNOR are hypoexpressed in elderly, but not in exceptionally long-lived, humans. Real-time PCR assays of GSNOR (A) and TET1 expression (B) in PBMCs obtained from humans of different ages, including long-lived individuals (95–101 y of age), are shown. Values are expressed as fold change relative to a young group (18–30 y of age) after normalizing to two different internal standards (actin and L34), and they represent mean ± SEM of n ≥ 12 individuals per experimental point analyzed in triplicate. P values are shown in the graph (Student’s t test). (C) Correlation between the paired mRNA levels of GSNOR and TET1 analyzed by real-time PCR in PBMCs from humans of different ages (as shown in A and B). The linear regression and Pearson correlation coefficient were calculated using GraphPad Prism 6.0 (GraphPad Software, Inc.). R2 = 0.1418, P = 0.0236.