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GluK1/Neto1 receptor complex interacts with PSD-95 through PDZ-binding motifs. HEK293T cells were transfected with expression plasmids of GFP-tagged PSD-95 together with wild-type HA-tagged GluK1 and Neto1 or respective mutants deleting the last for amino acids (HA-GluK1Δ4 and Neto1Δ4) as indicated. Western blot was performed to analyze the levels of PSD-95 and GluK1 in the HA immunoprecipitates (IP) and whole-cell lysate (Input) with indicated antibodies.
