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. Author manuscript; available in PMC: 2018 Aug 21.
Published in final edited form as: Nature. 2018 Feb 21;555(7694):54–60. doi: 10.1038/nature25741

Figure 3. Predicted fate couplings confirmed by single cell fate assays.

Figure 3

a Schematic of single cell liquid cultures, measuring clonal output with the indicated antibodies.

b Lineage output (left) and size (right) of each clone (rows) as in (a).

c Lineage co-occurrences in (b), computed by comparing the number of clones producing a pair of fates to the number expected following randomization.

d Fraction of bipotent erythroid-basophil (EBa) clones in P1-P5, containing E and Ba cells, but no other fates. Individual points and error bars show the expectation value and standard error from independent single cell sorting experiments. Bars represent the mean of n=two (P1, P2, P3) or n=three (P5) independent experiments; a single experiment was performed for P4 and CD55-.

e Cell state hierarchy based on fate co-occurrence in single-cell cultures.