Skip to main content
. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Dev Biol. 2018 Mar 3;437(1):17–26. doi: 10.1016/j.ydbio.2018.02.021

Figure 4. Vangl1 and Vangl2 are required for proper stereociliary bundle orientation in the saccular maculae.

Figure 4

(A) An overview of the P2 mouse saccular maculae showing the relative position (framed region) of β2-SPECTRIN labeled hair cells analyzed in this study and the approximate location of the LPR (yellow line). Analysis was restricted to this region because the LPR is less circuitous here relative to other parts of the sensory epithelia. The adjacent lateral boundary of the sensory epithelia (dashed white line) serves as a reference for stereociliary bundle orientation measures. (B) Vestibular hair cells from the saccule of littermate control mice labeled with β2-SPECTRIN and flanking the LPR (yellow line). (C) Schematic illustration of stereociliary bundle orientations for hair cells imaged in ‘B’. Occasional hair cells with stereociliary bundle orientations that do not match their neighbors are indicated (asters). (D) Vestibular hair cells from the saccule of Pax2-Cre; V1/V2 CKO mice labeled with β2-SPECTRIN. (E) Schematic illustration of stereociliary bundle orientations for hair cells imaged in ‘D’. (F) The orientation of individual stereociliary bundles graphed on circular histograms for all hair cells analyzed in the saccule Pax2-Cre; V1/V2 CKO and littermate control mice. For these histograms, 90° is oriented toward the lateral border of the sensory epithelia as illustrated in ‘A’ and 270° is oriented away from this border. The total number of hair cells represented by each histogram (n) is shown, and bin width is 5°.