A) Kaplain-Meier survival curves of DF-BM355/-bearing mice show improved
survival by treatment with dual PI3K + mTOR inhibition [BKM120 (30 mg/kg, PO) +
RAD001 (7.5 mg/kg, QD)] but not with single agent PI3K, HER2 (lapatinib, 100
mg/kg PO) or mTOR drug inhibition (n=5–18). Adapted from Ni et al, Nat
Med 2016. B) When implanted in the mammary gland (MG), DFBM-355 tumor growth
inhibition by PI3K+mTOR (BKM+RAD) is equivalent to PI3K+HER2 (BKM+LAP) or
HER2+mTOR (LAP+RAD) inhibition. Data shown as mean ± SEM,
(n=6–8). Difference tested by two-way ANOVA, followed by
Dunnett’s multiple comparison test ** P < 0.01.
C) AKT/mTOR gene signature expression is reduced in DFBM-355 implanted in
mammary gland compared to the brain. Data are represented as mean ± SD
(n = 3–5 per group; difference tested by
t-test. P = 0.0004).