Table 1.

Comparison of recently generated HSC-specific fluorescent reporters

Gene	Method	HSC expression	Expression in other BM populations	Labeled HSC observations	Ref.
Ctnnal1 (α-catulin)	Knock-in: GFP	50% LSK CD48⁻ CD150⁺	Minor (<1%) fractions of cKit⁻ or cKit⁺ SSC^hi	Localization of cells in BM sinusoids away from bone surface, no distinct niche for quiescent vs. proliferating HSCs	[30]
Fgd5	Knock-in: mCherry, zsGreen	80% LSK CD48⁻ CD150⁺ (mCherry)	Small (5–10%) fractions of progenitors, endothelial cells	All reconstituting activity lies within the labeled population	[34]
Gprc5c	BAC Tg: EGFP	28% LSK CD34⁻ CD48⁻ CD135⁻ CD150⁺	Small (<5%) fractions of progenitors	Enriched for dormant cells, improved outcomes upon transplantation	[31]
Hoxb5	Knock-in: tri-mCherry	22% LSK CD34− CD135− CD150+	Small (5%) fractions of LSK CD34⁺ CD135⁻ CD150⁺, unquantified fractions of Lin⁺ or Lin⁻ cKit⁺	Enriched for cells that have enhanced reconstitution of both primary and secondary hosts, proximity to VE-cadherin⁺ cells in BM	[33]
Pdzk1ip1	BAC Tg: EGFP	27% LSK CD48⁻ CD150⁺ (EGFP)	4% granulocytes and minor (<1%) fractions of progenitors (EGFP)	Enriched for cells that serially reconstitute (EGFP)	[37]
Tek (Tie2)	Tg: Green Lantern GFP	5% LSK CD34⁻ CD48⁻ CD135⁻ CD150⁺	Minor fractions of other primitive populations, endothelial cells	Enriched for cells that have enhanced reconstitution and undergo mitophagy	[35]
Vwf	BAC Tg: EGFP, tdTomato	60% LSK CD34⁻ CD48⁻ CD150⁺	Nearly all MkP and platelets, 50% of endothelial cells	Increased contribution to platelet production	[32, 36]