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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Dev Biol. 2018 Mar 5;437(1):27–40. doi: 10.1016/j.ydbio.2018.03.003

Figure 9. Vxn and p27Xic1 work together to regulate cell cycle exit and neurogenesis.

Figure 9

(A-E) Injection of 1ng GFP mRNA at 2CS (A) does not change pH3 labeling (B), while co-injection of 1ng vxn mRNA causes significant reduction of pH3 labeling on the injected side (bracket in D; E). * = p<0.01.

(F, G) Co-injection of p27Xic1 morpholino (p27MO) with vxn mRNA rescues the pH3 levels (F, *p< 0.001), and prevents expanded N-tubulin (G) on the injected side.

(H) Injection of p27Xic1 mRNA at 2CS promotes expanded N-tubulin, which is reversed by co-injection of 20ng vxn MO-B.

(I) Western blot (WB) to detect Myc-Neurog2 when expressed alone or together with vxn or p27Xic1. β-actin was detected as a loading control.

For all experiments, mRNA encoding GFP was co-injected to label the injected side (right).