Table I.
cDNA Library | ESTb | Best Match | P Valuec | Redundancyd | Representatione | G + C | Source |
---|---|---|---|---|---|---|---|
% | |||||||
Infected soybean | 3-10E-HA | Pathogenesis-related protein 1 | 10−119 | 10 | HA | 42 | G. maxf |
3-4G-HA | Formate dehydrogenase | 10−127 | 7 | HA | 67 | P. sojaef | |
4-5G-HA | Alcohol dehydrogenase | 10−67 | 7 | HA | 64 | P. sojaef | |
4-6H-HA | Stress-induced PR protein | 10−87 | 6 | HA | 45 | G. maxg | |
4-7B-HA | ATP synthase, β-chain | 10−157 | 4 | HA, MY | 64 | P. sojaeg | |
8-6C-HA | Chalcone synthase | 10−210 | 4 | HA | 47 | G. maxg | |
5-2H-HA | 2-Phosphoglycerate dehydratase | 10−164 | 4 | HA, MY | 56 | P. sojaeg | |
2-11G-HA | Glu dehydrogenase | 10−53 | 4 | HA | 64 | P. sojaeg | |
Mycelium | 8-12D-MY | RIC1 protein | 10−32 | 8 | MY | ||
9-9D-MY | Unknown/hypothetical protein | 10−7 | 8 | MY | |||
11-9A-MY | Annexin | 10−27 | 7 | HA, MY | |||
7-4D-MY | Guanine nucleotide binding protein | 10−155 | 6 | HA, MY | |||
6-10C-MY | Unknown/hypothetical protein | 10−7 | 5 | MY | |||
4-9A-MY | Thioredoxin peroxidase | 10−76 | 4 | MY | |||
3-7A-MY | Superoxide dismutase | 10−55 | 4 | HA, MY | |||
Zoospore | 3-10A-ZO | Elicitin | 10−5 | 8 | ZO | ||
11-6F-ZO | Unknown/hypothetical protein | 10−9 | 8 | ZO | |||
4-2B-ZO | Unknown/hypothetical protein | 10−21 | 8 | ZO | |||
6-5E-ZO | ABC transporter | 10−31 | 7 | ZO | |||
9-2D-ZO | GTP cyclohydrolase | 10−67 | 6 | ZO | |||
10-7B-ZO | Ribonucleotide reductase | 10−128 | 6 | ZO | |||
5-7D-ZO | Actin A | 10−219 | 5 | HA, MY, ZO | |||
4-2F-ZO | Membrane glycoprotein | 10−10 | 5 | ZO | |||
7-3H-ZO | HAM34 protein | 10−18 | 5 | ZO | |||
10-6E-ZO | Mucin | 10−14 | 4 | ZO | |||
3-5A-ZO | Unknown/hypothetical protein | 10−9 | 4 | ZO | |||
4-1H-ZO | Transcription factor protein | 10−4 | 4 | ZO |
Not including ribosomal proteins. Total no. of ESTs in each library: 1,002 infected plant (HA); 1,002 mycelium (MY); and 1,031 zoospore (ZO).
Representative EST clone from assembly contig.
P value from BLASTX search using entire contiguous sequence resulting from assembly of ESTs.
No. of ESTs from an individual source library that assembled into a contiguous sequence.
Representation of transcript in all three source libraries.
Determined by hybridization analysis.
Predicted from representation, BLASTX match, and percentage G + C content.