Figure 7.

Registration quality of whole mouse histology to color episcopic cryo-image. A whole mouse histological section was obtained with an adhesive film technique and registered to the corresponding color cryo-image (a). A checkerboard display (b) shows that many edges line up in the two registered images. A high resolution checkerboard with 400 μm on a side was used to roughly estimate registration accuracy by manually measuring distances between edges. Images (c) and (d) show significant edges from cryo-images (black) and histology (white) after affine (c) and nonrigid (d) registration. Pixels colored red indicate an overlay of both black and white edges. The amount of red pixels indicates excellent overlap of edges and improvement with nonrigid registration. Bar is 2750 μm.