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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2018 Jan 16;1863(4):409–419. doi: 10.1016/j.bbalip.2018.01.008

Fig. 3.

Fig. 3

Overexpression of AK079912 induces the browning of white adipocytes. (A) Relative levels of AK079912 in iWAT-derived stromal vascular fraction (svf) cells after infected with control and AK079912 overexpression (OE) adenovirus, n = 6 (3 biological replicates for each independent experiment). (B–C) Oil Red O staining (B) and lipid content (C) of adipocytes differentiated from iWAT-derived svf cells infected with control and OE adenovirus. (D–F) Relative mRNA levels of adipogenic (D), browning (E) and lipolysis (F) related genes in control and OE white adipocytes (n = 6, 3 biological replicates for each independent experiment). (G) Protein levels of aP2 and Ucp1 in control and OE cells (n = 4, 2 biological replicates for each independent experiment). Scale bar: 100 μm, Error bar: Mean ± SEM, *P < 0.05, **P < 0.01.