Figure 3.

Localization of p-pmsr and c-pmsr mRNA and proteins in various tissues. P and C are northern blots probed with specific cDNA probes for the plastidial (1.1 kb) and cytosolic (0.9 kb) forms of pmsr, respectively. Lanes contain 10 μg of total RNA. L, Loading control (same RNA stained with ethidium bromide); W, western blot probed with polyclonal antibodies that recognized both the mature pPMSR protein at 28 kD and the cPMSR protein at 27 kD. Lane 1, Roots; lane 2, base of stem; lane 3, upper stem; lane 4, rosette leaves; lane 5, cauline leaves; lane 6, flower buds; lane 7, open flowers; lane 8, siliques (mid-maturation).