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Enzymatic activity of recombinant Arabidopsis pPMSR and cPMSR
| Dithiothreitol | Protein | Substrate 1b | Assay 1 |
|---|---|---|---|
| pmol MetSO reduced | |||
| − | − | − | 0 |
| − | − | − | 0 |
| + | − | + | 2 |
| + | pPMSR | + | 69.0 |
| − | pPMSR | + | 4.8 |
| + | cPMSR | + | 67.3 |
| − | cPMSR | + | 5.0 |
| + | BSAa | + | 2.0 |
| Dithiothreitol | Protein | Substrate 2c | Assay 2 |
|---|---|---|---|
| % of substrate | |||
| − | − | + | 0 |
| + | − | + | 3 |
| + | pPMSR | + | 75 |
| − | pPMSR | + | 4 |
| + | cPMSR | + | 75 |
| − | cPMSR | + | 5 |
| + | cPMSR, boiled | + | 3 |
BSA, Bovine serum albumin.
Substrate 1, N-Acetyl-[3H]-MetSO.
Substrate 2, Bovine α-1 proteinase inhibitor.
