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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2017 Dec 14;75(4):164–173. doi: 10.1002/cm.21425

Table 2.

Diffusion coefficients

Method, conditions Diffusing species D (μm2/s)
Mean ±SD		 Number of cells or measurements
Cytoplasm of live cells
FRAP live interphase cells mEGFP monomer 22 9
FCS live interphase cells mEGFP monomer 27. ± 8.5 20
FRAP live interphase cells mEGFP-Myo2 3 8
FRAP live mitotic cells mEGFP-Myo2 2.5 8
FCS live interphase cells mEGFP-Myo2 1.8 ± 0.5 20
FCS live mitotic cells mEGFP-Myo2 1.9 ± 0.5 20
Diluted cell extracts
FCS diluted cell extract 50 mM KCl mEGFP monomer 76.7 ± 23.0 60
FCS diluted cell extract 50 mM KCl mEGFP-Myo2 37.8 ± 9.4 60
FCS diluted cell extract 200 mM KCl mEGFP-Myo2 29.7 ± 3.4 60
FCS diluted cell extract 400 mM KCl mEGFP-Myo2 30.0 ± 4.7 60

In FCS experiments, 10 measurements were acquired and averaged from single points in cells or cell extracts. The samples consisted of 20 cells or 20 points in each of 3 cell extracts.