Table 2.
Passive membrane properties of directly differentiated neurons from control hiPSC lines 210 and 173.
| Line | Days post plating
|
|||||
|---|---|---|---|---|---|---|
| 4–5 | 10–11 | 15–16 | 21–24 | 35–38 | ||
| Rm (GΩ) | 210 | 2.6 ± 0.4 | 2.5 ± 0.2 | 2.2 ± 0.4 | 1.6 ± 0.0.5 | 1.4 ± 0.4 |
| 4(36) | 5(51) | 5(81) | 7(131) | 4(101) | ||
| 173 | 2.7 ± 0.6 | 2.3 ± 0.4 | 1.9 ± 0.3 | 1.7 ± 0.2 | 1.2 ± 0.2 | |
| 4(39) | 6(47) | 6(48) | 6(65) | 6(52) | ||
| RMP (mV) | 210 | −53.5 ± 8.4 | −50.8 ± 6.4 | −55.1 ± 4.5 | −54.1 ± 3.6 | −53.4 ± 4.1 |
| 4(36) | 5(51) | 5(81) | 7(131) | 4(101) | ||
| 173 | −50.2 ± 2.3 | −50.0 ± 3.2 | −54.7 ± 5.0 | −50.1 ± 2.5 | −48.6 ± 2.7 | |
| 4(39) | 6(47) | 6(48) | 6(65) | 6(52) | ||
| Cm (pF) | 210 | 25.5 ± 3.0 | 25.2 ± 3.2 | 33.0 ± 5.2a | 37.0 ± 8.5b | 43.5 ± 15.9 |
| 4(36) | 5(51) | 5(81) | 7(131) | 4(101) | ||
| 173 | 15.1 ± 1.8 | 19.0 ± 2.2 | 19.1 ± 3.2a | 23.4 ± 1.8b | 34.4 ± 3.7 | |
| 4(31) | 6(58) | 6(50) | 6(63) | 6(69) | ||
Data reported as mean ± SD with number of individual platings (total number of neurons).
a
p < 0.01.
b
p < 0.001, denotes significance between cell lines at given time point post plating (two-way ANOVA with Bonferroni post hoc test).