Table 3.
Kinetic parameters of the wild-type human SULT1A3 and allozymes with epinephrine as a substrate.
| SULT1A3 allozymes | Km (μM) | Vmax (nmol/min/mg) | Vmax/Km |
|---|---|---|---|
| 1A3-WT1 | 9.16 ± 1.81 | 35.30 ± 1.82 | 3.85 |
| 1A3-T7P | 8.90 ± 0.89 | 33.72 ± 0.87 | 3.79 |
| 1A3-S8P | 9.73 ± 0.47 | 35.60 ± 0.45 | 3.66 |
| 1A3-R9C | 9.44 ± 0.40 | 30.65 ± 1.34 | 3.25 |
| 1A3-P10L | 10.86 ± 0.48 | 38.66 ± 0.46 | 3.56 |
| 1A3-V15M | 9.05 ± 1.22 | 34.38 ± 1.20 | 3.80 |
| 1A3-V18F | 9.62 ± 1.08 | 30.69 ± 0.91 | 3.19 |
| 1A3-P101L | 8.83 ± 1.23 | 34.97 ± 1.26 | 3.96 |
| 1A3-P101H | 9.67 ± 1.71 | 33.95 ± 1.58 | 3.51 |
| 1A3-R144C | 11.19 ± 1.69 | 34.70 ± 1.42 | 3.10 |
| 1A3-K234N | 8.58 ± 1.24 | 29.30 ± 1.08 | 3.41 |
| 1A3-N235T | 200.1 ± 64.51 | 25.85 ± 6.05 | 0.13 |
| 1A3-S290T | 11.12 ± 0.45 | 29.60 ± 1.27 | 2.66 |
1
Wild-type human SULT1A3.