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. Author manuscript; available in PMC: 2019 Apr 15.
Published in final edited form as: Acta Biomater. 2018 Mar 8;71:118–131. doi: 10.1016/j.actbio.2018.03.003

Fig. 4.

Fig. 4

Decellularized ECM suppressed the formation of the F-actin ring in osteoclasts. (A) After a five-day induction, the F-actin rings were labeled with phalloidin and the nuclei were counterstained with DAPI. Scale bar = 200 μm. (B) The area of F-actin rings of the ECM-cultured osteoclasts was significantly smaller than the TCPS-cultured cells. (C) The percent nuclei in F-actin was expressed as an index for fusion efficiency, which was calculated as nuclei within F-actin rings divided by total nuclei. A significantly decreased fusion efficiency was observed in the ECM-cultured BMMs. Multinucleated osteoclasts were counted in at least ten randomly chosen fields of view (FOV). Values are presented as the mean ± S.E.M. of four independent experiments (n = 4). Statistically significant differences are indicated by * p < 0.05 or ** p < 0.01 between the indicated groups.