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. Author manuscript; available in PMC: 2019 Apr 15.
Published in final edited form as: Acta Biomater. 2018 Mar 8;71:118–131. doi: 10.1016/j.actbio.2018.03.003

Fig. 7.

Fig. 7

Supplementation with exogenous H2O2 partially rescued ECM-inhibited osteoclastogenesis. BMMs were cultured on cell-derived ECM and induced toward osteoclasts with or without the addition of 20 μM H2O2. (A) Multinucleated osteoclasts were positively stained for TRAP and cell nuclei were counterstained with DAPI (three replicates for each treatment). Scale bar = 200 μm. (B–C) Quantification of the cell number of TRAP-positive osteoclasts with greater than 3 nuclei (B) and with 8 or more nuclei (C). TRAP-positive osteoclasts were counted in at least ten randomly chosen fields of view (FOV). (D–F) The mRNA levels of TRAP (D), CTSK (E), and OSCAR (F) were measured by real-time RT-PCR. Values are presented as the mean ± S.E.M. of four independent experiments (n = 4). Statistically significant differences are indicated by * p < 0.05 or ** p < 0.01 between the indicated groups.