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. Author manuscript; available in PMC: 2019 May 1.

Published in final edited form as: Biochem Pharmacol. 2017 Dec 7;151:201–213. doi: 10.1016/j.bcp.2017.12.003

A_2BAR-mediated ERK1/2 activity in 1321N1 astrocytoma cells. A. Time-course of NECA-suppressed ERK1/2 activity. B. Effect of Gq/11 inhibitor UBO-QIC, Gi inhibitor PTX and Gs downregulator CTX on NECA-suppressed ERK1/2 activity. Cells were pretreated with UBO-QIC for 30 min or PTX and CTX overnight before the addition of agonist and incubated for another 10 min. *Significantly different from control (P<0.05). #Significantly different from NECA group (P<0.05). C. Comparison of the suppression of ERK1/2 activity by adenylyl cyclase stimulator forskolin and agonist NECA, and the effects of PKA and EPAC inhibitors on NECA-induced suppression of ERK1/2 activity. Control and NECA+GO groups were expressed as 100% and 0%, respectively. *Significantly different from control (P<0.05, unpaired t test). ^#Significantly different from NECA group (P<0.05, One-Way ANOVA followed by post hoc test (Bonferroni)). D. Concentration-response of ERK1/2 activity suppressed by four A_2BAR agonists. Results are expressed as mean ± SD from at least three independent experiments performed in duplicate or triplicate.

A_2BAR-mediated ERK1/2 activity in 1321N1 astrocytoma cells. A. Time-course of NECA-suppressed ERK1/2 activity. B. Effect of Gq/11 inhibitor UBO-QIC, Gi inhibitor PTX and Gs downregulator CTX on NECA-suppressed ERK1/2 activity. Cells were pretreated with UBO-QIC for 30 min or PTX and CTX overnight before the addition of agonist and incubated for another 10 min. *Significantly different from control (P<0.05). #Significantly different from NECA group (P<0.05). C. Comparison of the suppression of ERK1/2 activity by adenylyl cyclase stimulator forskolin and agonist NECA, and the effects of PKA and EPAC inhibitors on NECA-induced suppression of ERK1/2 activity. Control and NECA+GO groups were expressed as 100% and 0%, respectively. *Significantly different from control (P<0.05, unpaired t test). ^#Significantly different from NECA group (P<0.05, One-Way ANOVA followed by post hoc test (Bonferroni)). D. Concentration-response of ERK1/2 activity suppressed by four A_2BAR agonists. Results are expressed as mean ± SD from at least three independent experiments performed in duplicate or triplicate.