A2BAR-mediated ERK1/2 activity in 1321N1 astrocytoma cells. A.
Time-course of NECA-suppressed ERK1/2 activity. B. Effect of Gq/11 inhibitor
UBO-QIC, Gi inhibitor PTX and Gs downregulator CTX on NECA-suppressed ERK1/2
activity. Cells were pretreated with UBO-QIC for 30 min or PTX and CTX overnight
before the addition of agonist and incubated for another 10 min.
*Significantly different from control (P<0.05).
#Significantly different from NECA group (P<0.05). C. Comparison of
the suppression of ERK1/2 activity by adenylyl cyclase stimulator forskolin and
agonist NECA, and the effects of PKA and EPAC inhibitors on NECA-induced
suppression of ERK1/2 activity. Control and NECA+GO groups were
expressed as 100% and 0%, respectively. *Significantly
different from control (P<0.05, unpaired t test).
#Significantly different from NECA group (P<0.05, One-Way
ANOVA followed by post hoc test (Bonferroni)). D. Concentration-response of
ERK1/2 activity suppressed by four A2BAR agonists. Results are
expressed as mean ± SD from at least three independent experiments
performed in duplicate or triplicate.