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Intracellular calcium mobilization in 1321N1 astrocytoma cells. Cells were plated in 96-well black plates with clear bottom and were grown overnight. Calcium 6 dye was loaded as instructed by kit manual and incubated for 60 at room temperature before the addition of agonists carbachol or NECA. Each data point represents the mean ± SD from three experiments performed in triplicate.
