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. Author manuscript; available in PMC: 2019 Apr 15.
Published in final edited form as: Acta Biomater. 2018 Mar 7;71:444–459. doi: 10.1016/j.actbio.2018.02.037

Figure 2.

Figure 2

(A) Elastic modulus of extracellular matrix derived from five different glaucomatous TM donors cultured in the absence (EtOH vehicle control) presence of 100 nM dexamethasone (Dex). At least 7–10 random locations were probed with 5 force curves obtained at each location. Results are mean ± standard deviation.*p<0.05, **p<0.01, ***p<0.001, t-test (with Bonferroni correction) compared with vehicle control (EtOH). (B) Comparison of elastic moduli of cell derived matrices obtained from glaucomatous (5 donors) and non-glaucomatous (3 donors) TM cell cultures. Data are the mean ± standard deviation of all force curves obtained for all donors at all locations. ***p<0.001, t-test compared with normal (non-glaucomatous, and ##p<0.01, t-test compared with vehicle control (EtOH). (C) Representative image of fibronectin organization in the matrix deposited by non-glaucomatous and glaucomatous cells. (D) Matrix proteins from glaucomatous and non-glaucomatous donors cluster differently. Heat map and dendrogram based on mean differences in protein expression between each group. Data are from 3 donors for each group.