Figure 7. The effects of purines on USMC Ca²⁺ transients.

A, relative expression of P2X receptor genes in FACS sorted and unsorted urethral cells from SMC‐eGFP mice determined by qPCR analysis, normalized to Gapdh expression (n = 4). B, relative expression of P2Y receptor genes in FACS sorted and unsorted urethral cells from SMC‐eGFP mice determined by qPCR analysis, normalized to Gapdh expression (n = 4). C, representative ST map showing the effect of 10 μm ATP on Ca²⁺ transient firing in USMCs. Da–d, summary data showing the effect of 10 μm ATP on USMC Ca²⁺ transient frequency (a), amplitude (b), duration (c) and spatial spread (d) (c = 5, n = 3). ns, not significant, ^** p < 0.01, ^**** p < 0.0001.