Figure 8. Ca²⁺ transients in USMCs rely on functional SR stores.

Aa and b, representative ST maps showing the effect of 10 μm thapsigargin on Ca²⁺ transient firing in USMCs. Ba–d, summary data showing the effect of 10 μm thapsigargin on USMC Ca²⁺ transient frequency (a), amplitude (b), duration (c) and spatial spread (d) (c = 4, n = 3). Ca and b, representative ST maps showing the effect of 10 μm CPA on Ca²⁺ transient firing in USMCs. Da–d, summary data showing the effect of 10 μm CPA on USMC Ca²⁺ transient frequency (a), amplitude (b), duration (c) and spatial spread (d) (c = 13, n = 5). E, relative expression of IP₃R genes in FACS sorted and unsorted urethral cells from SMC‐eGFP mice determined by qPCR analysis, normalized to Gapdh expression (n = 4). F, relative expression of RyR genes in FACS sorted and unsorted urethral cells from SMC‐eGFP mice determined by qPCR analysis, normalized to Gapdh expression (n = 4). ^* p < 0.05, ^** p < 0.01, ^**** p < 0.0001.