Aa and b, representative ST maps showing the effect of 100 μm tetracaine on Ca2+ transient firing in USMCs. Ba–d, summary data showing the effect of 100 μm tetracaine on USMC Ca2+ transient frequency (a), amplitude (b), duration (c) and spatial spread (d) (c = 18, n = 6). Ca and b, representative ST maps showing the effect of 100 μm ryanodine on Ca2+ transient firing in USMCs. Da–d, summary data showing the effect of 100 μm ryanodine on USMC Ca2+ transient frequency (a), amplitude (b), duration (c) and spatial spread (d) (c = 5, n = 14). *
p < 0.01, ***
p < 0.001, ****
p < 0.0001.