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. 2018 Apr 15;596(8):1433–1466. doi: 10.1113/JP275719

Table 1.

Gene primers used in qPCR analysis

Gene name Sequence (sense primer on top) GenBank accession no.
Gapdh

  • GCCGATGCCCCCATGTTTGTGA

  • GGGTGGCAGTGATGGCATGGAC

 NM_008084
Pdgfra

  • ATGACAGCAGGCAGGGCTTCAACG

  • CGGCACAGGTCACCACGATCGTTT

 NM_011058
Kit

  • CGCCTGCCGAAATGTATGACG

  • GGTTCTCTGGGTTGGGGTTGC

 NM_021099
Myh11

  • CTACACTGCGCAATACCACG

  • TGCCAGGATCTCATAGCGTT

 NM_013607
Uchl1

  • CGATGGAGATTAACCCCGAGATG

  • TTTTCATGCTGGGCCGTGAG

 NM_011670
Trpc1

  • CTGTGGATTATTGGGATGATTTGGTCA

  • CACTTTGAGGGCAAAGGTTGCCAA

 NM_011643
Trpc2

  • CTCCTCCTGGCTGGGCTTG

  • GGTGAAGCTGAGCATGCTGGTG

 NM_011644
Trpc3

  • CAGCCAACACGATATCAGATAAT

  • AAGTTCATAACGAAGGCTGGAGATATC

 NM_019510
Trpc4

  • CCATGACTGGTGGAATCTAAT

  • GGTGCCACATGTCCCATGATT

 NM_016984
Trpc5

  • CATCTCCCTGGTAGTGCTGCTG

  • CACCTTCATCAAAGTAACTCATCCAGAG

 NM_009428
Trpc6

  • GTGGTGCTTGCTGTTGTCATTG

  • CTTCAAATCTGTCAGCTGCATTCATGAC

 NM_013838
Tpsab1

  • TGTGGGACCGGATGTTGCTGAC

  • AGTCGGGGTGTGTGATGATCTGG

 NM_031187
Chrm1

  • GCAACGCCTCTGTCATGAAT

  • AACGAAGGAAACCAACCACG

 NM_001112697
Chrm2

  • GGTGTCTCCCAGTCTAGTGCAAGG

  • ATGTCTGCCTAGAGTTGTCATCTTTGGA

 NM_203491
Chrm3

  • TGTGGCCAGCAATGCTTCTGTCATGA

  • CCACAGGACAAAGGAGATGACCCAAG

 NM_033269
Chrm4

  • ATGTGTGACTGCCATCGAGA

  • GGATGAAGGCCAGCAGAATG

 NM_007699
Chrm5

  • GTCAAAGAGAGGAAAGCGGC

  • TAGCACAGCCAGTAACCCAA

 NM_205783
Adra1a

  • GCCCATTGGGTCCTTCTTCCCG

  • ACTCCTGGCTGGAGCATGGG

 NM_013461
Adra1b

  • AGTGCTCGGCTACTGGGTGC

  • TGACCAGCGTGGGGTACTGC

 NM_007416
Adra1d

  • CGTCCTGCCTCTGGGTTCTCT

  • AGCAGGGGTAGATGAGCGGGT

 NM_013460
Npr1

  • ACATGGTGGTATCAGGGCTC

  • GTGTGAATTCCAATGCGCAAG

 NM_008727
Npr2

  • GAAGGTGCGGAATGGTCAGA

  • CCTTCCTTGTTAAACCGGCG

 NM_173788
Prkg1a

  • AACTCCACAAATGCCAGTCG

  • GGTCGTGGAAGGACCTGTAG

 NM_001013833
Prkg1b

  • GATTTACAGTATGCGCTCCAGG

  • AGCTTCTGGATCAGTTCGTC

 NM_011160
P2ry1

  • ACCGAGGTGCCTTGGTCGGT

  • CCGGTCTTGGTCAGGGCACA

 NM_008772
P2ry2

  • AGCACCATCAATGGCACCTGGGA

  • CACGACGTTCAGGCACAACCC

 NM_008773
P2ry6

  • ACCGCACTGTGTGCTACGAC

  • CGGCGGGCCATGCGACAATA

 NM_183168
P2ry12

  • GGCTGACGTCACTGAACGCCTG

  • TCTCTTCGCTTGGTTCGCCACC

 NM_027571
P2ry13

  • CAACACCACTGGGATGCAGGGCT

  • GCTGGGGATGTGGACGAACACC

 NM_028808
P2ry14

  • ACCCTCCAAACCAGCCCTGC

  • AGGCCCATGAGAAAGTCAGCCA

 NM_133200
P2rx1

  • CCACGCTTCAAGGTCAACAGGC

  • GATACCAACCACCCCGCCCTTC

 NM_008771
P2rx2

  • TTGTGCATGGACAGGCAGGGAAA

  • ACTTGAGGGGTGCCTTGGGGT

 NM_153400
P2rx3

  • CTTCCGTGGGAGTGGGGACTGTT

  • GCCCCCGAGTCTGTGGACTGC

 NM_145526
P2rx4

  • TGACGCTGGTGTGCCAACGC

  • GGCAGAAGGGATCCGTCCGC

 NM_011026
P2rx5

  • TCCGTCTCAGGGGGAGAACGT

  • TCCCCAGCGTGACAGTCGGT

 NM_033321
P2rx6

  • GAGAGTGGTGCTGTGCCCAGGAAG

  • CGGAACACTGGGCAGTACGGA

 NM_011028
Itpr1

  • TTATCAGCACCTTAGGCTTGGTTGA

  • ATCTGTAGTGCTGTTGGCCCCG

 NM_010585
Itpr2

  • CAACCCAGGCTGCAAAGAGGTGA

  • AGGTCGTCCGAAGGAAAATGTGCT

 NM_019923
Itpr3

  • CTTTGGGGCTGGTGGATGACCGTTG

  • TGCAGCTTCTGCAGCAATACCACA

 NM_080553
Ryr1

  • GTCAGTTCGAGCCCTGCAGGAG

  • GCAACTCAGGTACATACGACTGTGT

 NM_009109
Ryr2

  • TCCCCCGGACCTGTCTATCTGC

  • GGCCTCCACCTTGAGCAGTCTTC

 NM_023868
Ryr3

  • TCCTCGTCAGTGTGTCCTCTGAAA

  • CATGGCCACCGAGTAAGTATCCTTC

 NM_177652
Orai1

  • GTTCACTTCTACCGCTCCCT

  • GTGCCCGGTGTTAGAGAATG

 NM_175423
Orai2

  • CACAAGGGCATGGATTACCG

  • CCCTGCTCAGGTAGAGCTTC

 NM_178751
Orai3

  • GGCTGAAGTTGTTCTGGTGG

  • TGGAAGGCTGTTGTGATGTG

 NM_198424