FIGURE 1.

Schematic representation of antibody-guided immunoPET/MR imaging of IPA in a neutropenic mouse model of disease. Twenty-four hours (–1 day) prior to infection with A. fumigatus, and administration of the PET tracer, neutropenia is induced in mice by the injection of 100 μg of the anti-Ly-6G/anti-Ly6C antibody RB6-8C5. At time 0, mice are infected by intratracheal injection with an A. fumigatus spore suspension and with simultaneous tail vein injection with a JF5-based PET tracer. The tracer shown is mAb JF5 conjugated to the positron emitter ⁶⁴Cu using the chelator NODAGA. Simultaneous PET/MR imaging of animals is then performed 3, 24, and 48 h after infection/tracer injection. Ex vivo bio-distribution and autoradiography are conducted after the last PET scan at 48 h. Adapted from Davies et al. (2017).