Figure 5.
Locked nucleic acid (LNA) mitigates draining lymph node (dLN) effector cell and cytokines. Female C57BL/6 mice were given either syn (BL6) or allo (C3H) tail skin grafts. Mice receiving allografts were given either LNA-466 (10 mg/kg) or LNA-Ctrl (10 mg/kg) intraperitoneally 1 day before skin transplantation and every third day after that until termination of the study. (A) Survival curve of mice receiving skin transplants and indicated LNA or controls; n = 4 (syngeneic), 7 (allogeneic + Ctrl), or 8 (allogeneic + LNA). dLNs were harvested 12 days after skin transplantation from indicated groups imaged in (B), and absolute cell counts were taken in (C). dLN cells were plated overnight in complete media and culture supernatants were harvested and subjected to enzyme-linked immunosorbent assay (ELISA) for TNFα (D) and IFNγ (E). Serum was taken upon sacrifice and subjected to ELISA for transforming growth factor (TGF)-β1 (F) and TGF-β2 (G). Dot plots of circulating cells double positive for FoxP3 and CD62L, data are gated on CD4+ cells (H), and quantified in (I). (J) Hematoxylin & eosin stains of grafts excised from mice upon sacrifice. Data are presented as mean ± SEM; n = at least 4 per group. *P < 0.05, **P < 0.005, ***P < 0.001, ****P < 0.0001 by ANOVA with Tukey’s multiple comparisons test.